多功能性奈米材料分析化學實驗室

 

 

     The overall of my research goal is to synthesize and characterize new nano-materials with an emphasis on their applications in nanotechnology and biotechnology such as catalysts, sensors, etc.

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     My current research is focused on developing functional nanomaterials and seeking their application in the field of Analytical Chemistry. Recently, we have three major research directions:

(1) Separation of nanomaterials such as nanomaterials, metal clusters and carbon dots by Capillary      Electrophoresis (CE) and High-Performance Liquid Chromatography (HPLC)

(2) Synthesis of new nanomaterials for Catalysis, Imaging and Probings

(3) Development of Plasmonic-ELISA skill and Plasmonic-Biosensors.

 

 

 

Past Works : 

 

（1）毛細管電泳線上濃縮與分離生物分子

                  於毛細管電泳中添加適當中性或帶電荷之聚合物溶液（高黏度、篩分大小分子和易塗覆於管壁等特性），發現具             有避免生物分子吸附於管壁上、控制電滲流的大小、可增加分離的解析度與線上濃縮分析物等優勢，相當適用於偵測             低濃度蛋白質等分析物，並且成功應用於檢測人體體液中極微量的生物樣品。目前成果已發表於：

 

1. Yu, C.-J.; Chang, H.-C.; Tseng, W.-L.* “On-Line Concentration of Proteins by Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis with Laser-Induced Native Fluorescence” Electrophoresis 2008, 29, 483-490.

2. Lin, C.-Y.; Yu, C.-J.; Chen, Y.-M.; Chang, H.-C.; Tseng, W.-L.* “Simultaneous separation of anionic and cationic proteins by capillary electrophoresis using high concentration of poly(diallydimethylammonium chloride) as an additive” J. Chromatogr. A 2007, 1165, 219-225.

3. Yu, C. –J.; Tseng, W. –L.* “Online concentration and separation of basic proteins using cationic polyelectrolyte in the presence of reversed electroosmotic flow” Electrophoresis 2006, 27, 3569-3577.

 

 

（2）金奈米粒子在毛細管電泳上的應用

                 以Didecyldimethylammonium bromide(DDAB)或Poly(diallyldimethylammonium chloride) (PDDAC)修飾之金奈米粒子             做為毛細管電泳之假靜相，發現具有增加蛋白質分離的效率、縮短分離時間、減少蛋白質吸附與同時分離酸鹼蛋白質             等優點，並且已成功應用於檢測人體體液與雞蛋白。目前成果已發表於：

 

      Yu, C. -J.; Su, C. –L.; Tseng, W. -L.* “Separation of Acidic and Basic Proteinsby Nanoparticle-Filled Capillary Electrophoresis”             Anal. Chem. 2006, 78, 8004-8010.

 

 

（3）奈米粒子感應器

               過去所開發的奈米粒子感測器有下主要有兩種策略，首先係利用目標分析物導致金奈米子聚集，造成表面電漿共振           波長紅位移，以聚集奈米粒子與分散奈米粒子消光的比率對目標分析物定量。此策略所開發的感測器如下：（1）透過           控制金離子溶液的pH，可合成出粒徑分布較窄表面修飾上Gallic acid的金奈米粒子，除了可以窄化奈米粒子之粒徑分佈           外，還可藉由比色法 ( Colorimetric assay ) 進行高靈敏度與高選擇性之 Pb2+ 偵測，本實驗室所開發之感測Pb(II)金奈米感         測器，偵測極限可達10 nM。；（2）探討 Mn2+ 於比色法，對表面吸附有單股寡核甘酸 Polythymine T33 之金奈米粒               子，與 Hg2+ 進行雜交時的影響，發現可以有效改善比色法之靈敏度並加速其顏色的改變；( 3 )Hg(II)與Ag(I)離子可於             Tween 20修飾之金奈米粒子的表面沉積，移除穩定試劑Tween 20，進而導致奈米粒子聚集。利用此現象並加入適當遮蔽           試劑可開發出感測Hg(II)Ag(I)離子之金奈米粒子感測器。（4）以 3-Mercaptopropionate acid 與 Adenosine monophosphate           同時修飾於金奈米粒子上，於高鹽類下以吸收法檢測汞離子；（5）以 Human serum albumin 修飾的金奈米粒子，透過靜         電荷吸引，選擇性偵測高 pI 之 Lysozyme，並且已成功應用於偵測雞蛋中的 Lysozyme。而第二類奈米感測器偵測策略係         利用螢光分子吸附於金奈米粒子表面，因螢光共振轉移而導致螢光分子螢光消光。當目標分子出現取代螢光分子時，將         使其螢光恢復。以上所敘述之奈米感測器其成果已發表於：

 

1. Lin, C.-Y.; Yu, C.-J.; Lin, Y.-H.; Tseng, W.-L.* “Colorimetric Sensing of Silver(Ⅰ) and Mercury (Ⅱ) Ions Based on an Assembly of Tween 20-Stabilized Gold Nanoparticles” Anal. Chem. 2010, 82, 6830-6837.

2. Yu, C.-J.; Lin, C.-Y.; Liu, C. H.; Cheng, T. L.; Tseng, W.-L.* “Synthesis of poly(diallyldimethylammonium chloride)-coated Fe3O4 nanoparticles for colorimetric sensing of glucose and selective extraction of thiol” Biosensors and Bioelectronics 2010, 26, 913-917.

3. Huang, K.-W.; Yu, C.-J.; Tseng, W.-L.*"Sensitivity Enhancement in the Colorimetric Detection of Lead(II) Ion Using Gallic Acid-Capped Gold Nanoparticles: Improving Size Distribution and Minimizing Interparticle Repulsion" Biosensors and Bioelectronics 2010, 25, 984-989.

4. Yu, C.-J.; Cheng, T.-L.; Tseng, W.-L.* “Effects of Mn2+ on Oligonucleotide-Gold Nanoparticle Hybrids for Colorimetric Sensing of Hg2+: Improving Colorimetric Sensitivity and Accelerating Color Change” Biosensors and Bioelectronics 2009, 25, 204-210.

5. Yu, C.-J.; Tseng, W.-L.* “Colorimetric detection of mercury (II) in a high-salinity solution using gold nanoparticles capped with 3-mercaptopropionate acid and adenosine monophosphate” Langmuir 2008, 24, 12717-12722.

6. Chen, Y.-M.; Yu, C.-J.; Cheng, T.-L.; Tseng, W.-L.* “Colorimetric Detection of Lysozyme based on Electrostatic Interaction with HumanSerum Albumin-Modified Gold Nanoparticles” Langmuir 2008, 24, 3654-3660.

 

 

（4）利用雷射脫附游離化質譜儀檢測生物分子

           藉由檸檬酸修飾之金奈米粒子做為基質，以雷射脫附游離化質譜儀來偵測高鹽類溶液下之生物分子，例如：膽固醇              類，以及真實樣品尿液中的尿素、肌酸酐、尿酸和葡萄糖等分析物。目前相關成果已發表於：

 

      Wu, H.-P.; Yu, C-J.; Lin, C.-Y.; Lin, Y.-H.; Tseng, W.-L.* “Gold nanoparticles as assisted matrices for the detection of biomolecules       in a high-salt solution through laser desorption/ionization mass spectrometry” J. Am. Soc. Mass Spectrom. 2009, 20, 875-882.

 

 

（5）鐵奈米粒子合成與應用

            我們發表一種簡單方法，僅需要利用共沉澱方式即可以一步合成poly(diallydimethylammonium chloride)(PDDA)修飾的           Fe3O4 NPs。此奈米粒子可透過靜電吸引力吸附glucose oxidase，形成一glucose oxidase與Fe3O4 NPs的複合物；當加入             glucose於此複合物時，glucose oxidase會催化glucose產生H2O2，此時溶液中2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic           acid) (ABTS) 會與H2O2反應，透過Fe3O4 NPs催化，氧化的ABTS在420 nm有相當強吸收。此glucose oxidase與Fe3O4 NPs         的複合物可重複偵測glucose，並且可應用於偵測血液中glucose。其成果已發表於：

 

1. Yu, C.-J.; Lin, C.-Y.; Liu, C. H.; Cheng, T. L.; Tseng, W.-L.* “Synthesis of poly(diallyldimethyl- ammonium chloride)-coated Fe3O4 nanoparticles for colorimetric sensing of glucose and selective extraction of thiol” Biosensors and Bioelectronics 2010, 26, 913-917.

2. Liu, C.-H.; Yu, C.-J.; Tseng, W.-L.* “Fluorescence assay of catecholamines based on the inhibition of peroxidase-like activity of Fe3O4 nanoparticles” Anal. Chim Acta 2012, 745, 143-148.

3. Yu, C.-J.; Wu, S.-M.; Tseng, W.-L.* “Magnetite Nanoparticle-Induced Fluorescence Quenching of Adenosine Triphosphate-BODIPY Conjugates: Application to Adenosine Triphosphate and Pyrophosphate Sensing” Anal. Chem. 2013.